A point mutation in Sec61alpha1 leads to diabetes and hepatosteatosis in mice.

ABSTRACT:

Objective

Type 2 diabetes is caused by both environmental and genetic factors. To better understand the genetic factors we used forward genetics to discover genes that have not previously been implicated in the development of hyperglycemia or diabetes.

Research design and methods

Offspring of ethylnitrosurea-mutagenized C57BL/6 mice were bred to homozygosity, maintained on high-fat diet, and screened for hyperglycemia. The phenotype in one diabetic family of mice was mapped among hybrid F2s with single nucleotide polymorphic markers, followed by candidate gene sequencing to identify the gene harboring the causative mutation. Subsequent analysis was done on wild-type, heterozygous, and homozygous mutant mice on a pure C57BL/6 background.

Results

Diabetes mapped to a po

SUBMITTER: Lloyd DJ 

PROVIDER: S-EPMC2809972 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature