A targetable fluorescent probe for imaging hydrogen peroxide in the mitochondria of living cells.

ABSTRACT: We present the design, synthesis, and biological applications of mitochondria peroxy yellow 1 (MitoPY1), a new type of bifunctional fluorescent probe for imaging hydrogen peroxide levels within the mitochondria of living cells. MitoPY1 combines a chemoselective boronate-based switch and a mitochondrial-targeting phosphonium moiety for detection of hydrogen peroxide localized to cellular mitochondria. Confocal microscopy and flow cytometry experiments in a variety of mammalian cell types show that MitoPY1 can visualize localized changes in mitochondrial hydrogen peroxide concentrations generated by situations of oxidative stress.

SUBMITTER: Dickinson BC 

PROVIDER: S-EPMC2810491 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature