Transient anomalous subdiffusion: effects of specific and nonspecific probe binding with actin gels.

ABSTRACT: When signaling molecules diffuse through the cytosol, they encounter a wide variety of obstacles that hinder their mobility in space and time. Some of those factors include, but are not limited to, interactions with mobile and immobile targets or obstacles. Besides finding a crowded environment inside the cell, macromolecules assemble into molecular complexes that drive specific biological functions adding additional complexity to their diffusion. Thus, simple models of diffusion often fail to explain mobility through the cell interior, and new approaches are needed. Here we used fluorescent correlation spectroscopy to measure diffusion of three molecules of similar size with different surface properties diffusing in actin gels. The fluorescent probes were (a) quantum dots, (b) yellow-gree

SUBMITTER: Sanabria H 

PROVIDER: S-EPMC2810870 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature