High-resolution NMR structure of an RNA model system: the 14-mer cUUCGg tetraloop hairpin RNA.

ABSTRACT: We present a high-resolution nuclear magnetic resonance (NMR) solution structure of a 14-mer RNA hairpin capped by cUUCGg tetraloop. This short and very stable RNA presents an important model system for the study of RNA structure and dynamics using NMR spectroscopy, molecular dynamics (MD) simulations and RNA force-field development. The extraordinary high precision of the structure (root mean square deviation of 0.3 A) could be achieved by measuring and incorporating all currently accessible NMR parameters, including distances derived from nuclear Overhauser effect (NOE) intensities, torsion-angle dependent homonuclear and heteronuclear scalar coupling constants, projection-angle-dependent cross-correlated relaxation rates and residual dipolar couplings. The structure calculations were pe

SUBMITTER: Nozinovic S 

PROVIDER: S-EPMC2811024 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature