Reconstitution experiments and gene deletions reveal the existence of two-component major cell wall channels in the genus Corynebacterium.

ABSTRACT: Two small polypeptides, PorA and PorH, are known to form cell wall channels in Corynebacterium glutamicum and in Corynebacterium efficiens. The genes coding for both polypeptides are localized in close proximity to one another between the genes coding for GroEl2 and a polyphosphate kinase (PKK2). In this study, we investigated the relationship of PorA and PorH to one another. The results suggested that the major cell wall channels of Corynebacterium glutamicum, Corynebacterium efficiens, and Corynebacterium diphtheriae need the obligatory presence of two distinct polypeptides, one of class PorA and one of class PorH, to form an active cell wall channel. Identification of genes coding for homologous proteins in the chromosome of Corynebacterium callunae suggested a similar result for this s

SUBMITTER: Barth E 

PROVIDER: S-EPMC2812442 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature