A comprehensive proteomics and transcriptomics analysis of Bacillus subtilis salt stress adaptation.

ABSTRACT: In its natural habitats, Bacillus subtilis is exposed to changing osmolarity, necessitating adaptive stress responses. Transcriptomic and proteomic approaches can provide a picture of the dynamic changes occurring in salt-stressed B. subtilis cultures because these studies provide an unbiased view of cells coping with high salinity. We applied whole-genome microarray technology and metabolic labeling, combined with state-of-the-art proteomic techniques, to provide a global and time-resolved picture of the physiological response of B. subtilis cells exposed to a severe and sudden osmotic upshift. This combined experimental approach provided quantitative data for 3,961 mRNA transcription profiles, 590 expression profiles of proteins detected in the cytosol, and 383 expression profiles of pro

SUBMITTER: Hahne H 

PROVIDER: S-EPMC2812467 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature