Enhanced display of lipase on the Escherichia coli cell surface, based on transcriptome analysis.
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ABSTRACT:
SUBMITTER: Baek JH
PROVIDER: S-EPMC2812984 | biostudies-literature | 2010 Feb
REPOSITORIES: biostudies-literature
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Enhanced display of lipase on the Escherichia coli cell surface, based on transcriptome analysis.
Baek Jong Hwan JH Han Mee-Jung MJ Lee Seung Hwan SH Lee Sang Yup SY
Applied and environmental microbiology 20091130 3
A cell surface display system was developed using Escherichia coli OmpC as an anchoring motif. The fused Pseudomonas fluorescens SIK W1 lipase was successfully displayed on the surface of E. coli cells, and the lipase activity could be enhanced by the coexpression of the gadBC genes identified by transcriptome analysis. ...[more]
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