Molecular detection of viable bacterial pathogens in water by ratiometric pre-rRNA analysis.
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ABSTRACT:
SUBMITTER: Cangelosi GA
PROVIDER: S-EPMC2812999 | biostudies-literature | 2010 Feb
REPOSITORIES: biostudies-literature
Publications
Molecular detection of viable bacterial pathogens in water by ratiometric pre-rRNA analysis.
Cangelosi Gerard A GA Weigel Kris M KM Lefthand-Begay Clarita C Meschke John S JS
Applied and environmental microbiology 20091130 3
Ratiometric pre-rRNA analysis (RPA) detects the replenishment of rRNA precursors that occurs rapidly upon nutritional stimulation of bacterial cells. In contrast to DNA detection by PCR, RPA distinguishes viable from inactivated bacteria. It exhibits promise as a molecular viability test for pathogens in water and other environmental samples. ...[more]
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