Deep RNA sequencing of L. monocytogenes reveals overlapping and extensive stationary phase and sigma B-dependent transcriptomes, including multiple highly transcribed noncoding RNAs.

ABSTRACT:

Background

Identification of specific genes and gene expression patterns important for bacterial survival, transmission and pathogenesis is critically needed to enable development of more effective pathogen control strategies. The stationary phase stress response transcriptome, including many sigmaB-dependent genes, was defined for the human bacterial pathogen Listeria monocytogenes using RNA sequencing (RNA-Seq) with the Illumina Genome Analyzer. Specifically, bacterial transcriptomes were compared between stationary phase cells of L. monocytogenes 10403S and an otherwise isogenic DeltasigB mutant, which does not express the alternative sigma factor sigmaB, a major regulator of genes contributing to stress response, including stresses encountered upon entry into stationary phase.<

SUBMITTER: Oliver HF 

PROVIDER: S-EPMC2813243 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature