Rickettsial ompB promoter regulated expression of GFPuv in transformed Rickettsia montanensis.

ABSTRACT:

Background

Rickettsia spp. (Rickettsiales: Rickettsiaceae) are Gram-negative, obligate intracellular, alpha-proteobacteria that have historically been associated with blood-feeding arthropods. Certain species cause typhus and spotted fevers in humans, but others are of uncertain pathogenicity or may be strict arthropod endosymbionts. Genetic manipulation of rickettsiae should facilitate a better understanding of their interactions with hosts.

Methodology/principal findings

We transformed a species never associated with human disease, Rickettsia montanensis, by electroporation with a TN5 transposon (pMOD700) containing green fluorescent protein (GFPuv) and chloramphenicol acetyltransferase (CAT) genes under regulation of promoters cloned from the Rickettsia rickettsii ompB ge

SUBMITTER: Baldridge GD 

PROVIDER: S-EPMC2813287 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature