Comparing the functional roles of nonconserved sequence positions in homologous transcription repressors: implications for sequence/function analyses.

ABSTRACT: The explosion of protein sequences deduced from genetic code has led to both a problem and a potential resource: Efficient data use requires interpreting the functional impact of sequence change without experimentally characterizing each protein variant. Several groups have hypothesized that interpretation could be aided by analyzing the sequences of naturally occurring homologues. To that end, myriad sequence/function analyses have been developed to predict which conserved, semi-conserved, and nonconserved positions are functionally important. These positions must be discriminated from the nonconserved positions that are functionally silent. However, the assumptions that underlie sequence analyses are based on experimental results that are sparse and usually designed to address different

SUBMITTER: Tungtur S 

PROVIDER: S-EPMC2813367 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature