Genome-wide dynamics of replication timing revealed by in vitro models of mouse embryogenesis.

ABSTRACT: Differentiation of mouse embryonic stem cells (mESCs) is accompanied by changes in replication timing. To explore the relationship between replication timing and cell fate transitions, we constructed genome-wide replication-timing profiles of 22 independent mouse cell lines representing 10 stages of early mouse development, and transcription profiles for seven of these stages. Replication profiles were cell-type specific, with 45% of the genome exhibiting significant changes at some point during development that were generally coordinated with changes in transcription. Comparison of early and late epiblast cell culture models revealed a set of early-to-late replication switches completed at a stage equivalent to the post-implantation epiblast, prior to germ layer specification and down-reg

SUBMITTER: Hiratani I 

PROVIDER: S-EPMC2813472 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature