Notch1 regulates the functional contribution of RhoC to cervical carcinoma progression.

ABSTRACT:

Background

The role of Notch signalling in human epithelial cancers is of immense interest. In this study, we examine the interplay between Notch signalling and RhoC, a well-established molecular factor in metastasis. By linking the function of Notch and RhoC, we further strengthen the notion that there is a pro-oncogenic role of Notch signalling in human cervical cancers.

Methods

RhoC protein expression in cervical carcinoma cell lines was assessed by western blotting. Using CaSki and SiHa cells (cervical carcinoma cells lines), we show that RhoC contributes to wound healing, invasion and migration, anoikis resistance, colony formation, in vitro tube formation and tumour formation. Immunohistochemical studies were carried out to assess the co-expression of RhoC, pAkt and No

SUBMITTER: Srivastava S 

PROVIDER: S-EPMC2813755 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature