Tunable detection sensitivity of opiates in urine via a label-free porous silicon competitive inhibition immunosensor.

ABSTRACT: Currently, there is need for laboratory-based high-throughput and reliable point-of-care drug screening methodologies. We demonstrate here a chip-based label-free porous silicon (PSi) photonic sensor for detecting opiates in urine. This technique provides a cost-effective alternative to conventional labeled drug screening immunoassays with potential for translation to multiplexed analysis. Important effects of surface chemistry and competitive binding assay protocol on the sensitivity of opiate detection are revealed. Capability to tune sensitivity and detection range over approximately 3 orders of magnitude (18.0 nM to 10.8 muM) was achieved by varying the applied urine specimen volume (100-5 muL), which results in systematic shifts in the competitive binding response curve. A detection r

SUBMITTER: Bonanno LM 

PROVIDER: S-EPMC2814343 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature