Differential expression of hERG1 channel isoforms reproduces properties of native I(Kr) and modulates cardiac action potential characteristics.

ABSTRACT:

Background

The repolarizing cardiac rapid delayed rectifier current, I(Kr), is composed of ERG1 channels. It has been suggested that two isoforms of the ERG1 protein, ERG1a and ERG1b, both contribute to I(Kr). Marked heterogeneity in the kinetic properties of native I(Kr) has been described. We hypothesized that the heterogeneity of native I(Kr) can be reproduced by differential expression of ERG1a and ERG1b isoforms. Furthermore, the functional consequences of differential expression of ERG1 isoforms were explored as a potential mechanism underlying native heterogeneity of action potential duration (APD) and restitution.

Methodology/principal findings

The results show that the heterogeneity of native I(Kr) can be reproduced in heterologous expression systems by differential

SUBMITTER: Larsen AP 

PROVIDER: S-EPMC2814852 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature