Timing-dependent actions of NGF required for cell differentiation.

ABSTRACT:

Background

Continuous NGF stimulation induces PC12 cell differentiation. However, why continuous NGF stimulation is required for differentiation is unclear. In this study, we investigated the underlying mechanisms of the timing-dependent requirement of NGF action for cell differentiation.

Methodology/principal findings

To address the timing-dependency of the NGF action, we performed a discontinuous stimulation assay consisting of a first transient stimulation followed by an interval and then a second sustained stimulation and quantified the neurite extension level. Consequently, we observed a timing-dependent action of NGF on cell differentiation, and discontinuous NGF stimulation similarly induced differentiation. The first stimulation did not induce neurite extension, wher

SUBMITTER: Chung J 

PROVIDER: S-EPMC2814856 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature