Methylation patterns of cell-free plasma DNA in relapsing-remitting multiple sclerosis.

ABSTRACT:

Background

There is growing interest for identification of new targets for biomarker development in multiple sclerosis (MS). The goal of this study was to compare the concentration and the methylation patterns of cell-free plasma DNA (cfpDNA) in patients with relapsing-remitting multiple sclerosis (RRMS) and healthy individuals.

Methods

Three 30-patient cohorts were examined: patients with RRMS, in either remission or exacerbation, and healthy individuals as controls. Concentration of cfpDNA was determined using a standard fluorometric assay. Patterns of methylation in 56 gene promoters were determined by a microarray-based assay (MethDet-56). The data were analyzed to identify statistically relevant differences among the study groups.

Results

The concentration of cfp

SUBMITTER: Liggett T 

PROVIDER: S-EPMC2815078 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature