A motif-based analysis of glycan array data to determine the specificities of glycan-binding proteins.

ABSTRACT: Glycan arrays have enabled detailed studies of the specificities of glycan-binding proteins. A challenge in the interpretation of glycan array data is to determine the specific features of glycan structures that are critical for binding. To address this challenge, we have developed a systematic method to interpret glycan array data using a motif-based analysis. Each glycan on a glycan array is classified according to its component sub-structures, or motifs. We analyze the binding of a given lectin to each glycan in terms of the motifs in order to identify the motifs that are selectively present in the glycans that are bound by the lectin. We compared two different methods to calculate the identification, termed intensity segregation and motif segregation, for the analysis of three well-cha

SUBMITTER: Porter A 

PROVIDER: S-EPMC2815654 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature