Identification of cardiac myosin-binding protein C as a candidate biomarker of myocardial infarction by proteomics analysis.

ABSTRACT: Acute myocardial infarction (AMI) is a common cause of death for which effective treatments are available provided that diagnosis is rapid. The current diagnostic gold standards are circulating cardiac troponins I and T. However, their slow release delays diagnosis, and their persistence limits their utility in the identification of reinfarction. The aim was to identify candidate biomarkers of AMI. Isolated mouse hearts were perfused with oxygenated protein-free buffer, and coronary effluent was collected after ischemia or during matched normoxic perfusion. Effluents were analyzed using proteomics approaches based on one- or two-dimensional initial separation. Of the 459 proteins identified after ischemia with one-dimensional separation, 320 were not detected in the control coronary efflue

SUBMITTER: Jacquet S 

PROVIDER: S-EPMC2816024 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature