Coherent activation of a synthetic mammalian gene network.

ABSTRACT:

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A quantitative analysis of naturally-occurring regulatory networks, especially those present in mammalian cells, is difficult due to their high complexity. Much simpler gene networks can be engineered in model organisms and analyzed as isolated regulatory modules. Recently, several synthetic networks have been constructed in mammalian systems. However, most of these engineered mammalian networks have been characterized using steady-state population level measurements. Here, we use an integrated experimental-computational approach to analyze the dynamical response of a synthetic positive feedback network in individual mammalian cells. We observe a switch-like activation of the network with variable delay times in individual cells. In agreement with a stochastic model of t

SUBMITTER: Longo DM 

PROVIDER: S-EPMC2816225 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature