Loss of IGF2 imprinting is associated with abrogation of long-range intrachromosomal interactions in human cancer cells.

ABSTRACT: Nuclear architecture and chromatin geography are important factors in the regulation of gene expression, as these components may play a vital epigenetic role both in normal physiology as well as in the initiation and progression of malignancies. Using a modification of the chromosome conformation capture (3C) technique, we examined long-range chromatin interactions of the imprinted human IGF2 gene. We demonstrate that numerous intrachromosomal interactions occur along both parental alleles in normal tissues, where the IGF2 is paternally expressed, as well as in normal liver where gene expression is biallelic. Long-range and allele-specific interactions occur between the IGF2/H19 imprinting control region-1 (ICR1) and ICR2, a region which regulates an imprinted gene cluster nearly a megabas

SUBMITTER: Vu TH 

PROVIDER: S-EPMC2816615 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature