Analysis of high-throughput sequencing and annotation strategies for phage genomes.

ABSTRACT:

Background

Bacterial viruses (phages) play a critical role in shaping microbial populations as they influence both host mortality and horizontal gene transfer. As such, they have a significant impact on local and global ecosystem function and human health. Despite their importance, little is known about the genomic diversity harbored in phages, as methods to capture complete phage genomes have been hampered by the lack of knowledge about the target genomes, and difficulties in generating sufficient quantities of genomic DNA for sequencing. Of the approximately 550 phage genomes currently available in the public domain, fewer than 5% are marine phage.

Methodology/principal findings

To advance the study of phage biology through comparative genomic approaches we used marine cya

SUBMITTER: Henn MR 

PROVIDER: S-EPMC2816706 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature