Regulation of gene expression in hepatic cells by the mammalian Target of Rapamycin (mTOR).

ABSTRACT:

Background

We investigated mTOR regulation of gene expression by studying rapamycin effect in two hepatic cell lines, the non-tumorigenic WB-F344 cells and the tumorigenic WB311 cells. The latter are resistant to the growth inhibitory effects of rapamycin, thus providing us with an opportunity to study the gene expression effects of rapamycin without confounding effects on cell proliferation.

Methodology/principal findings

The hepatic cells were exposed to rapamycin for 24 hr. Microarray analysis on total RNA preparations identified genes that were affected by rapamycin in both cell lines and, therefore, modulated independent of growth arrest. Further studies showed that the promoter regions of these genes included E-box-containing transcription factor binding sites at highe

SUBMITTER: Jimenez RH 

PROVIDER: S-EPMC2816708 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature