Comprehensive identification and modified-site mapping of S-nitrosylated targets in prostate epithelial cells.

ABSTRACT:

Background

Although overexpression of nitric oxide synthases (NOSs) has been found associated with prostate diseases, the underlying mechanisms for NOS-related prostatic diseases remain unclear. One proposed mechanism is related to the S-nitrosylation of key regulatory proteins in cell-signaling pathways due to elevated levels of NO in the prostate. Thus, our primary objective was to identify S-nitrosylated targets in an immortalized normal prostate epithelial cell line, NPrEC.

Methodology/principal findings

We treated NPrEC with nitroso-cysteine and used the biotin switch technique followed by gel-based separation and mass spectrometry protein identification (using the LTQ-Orbitrap) to discover S-nitrosylated (SNO) proteins in the treated cells. In parallel, we adapted a pe

SUBMITTER: Lam YW 

PROVIDER: S-EPMC2816712 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature