Characterization of Notch1 antibodies that inhibit signaling of both normal and mutated Notch1 receptors.

ABSTRACT:

Background

Notch receptors normally play a key role in guiding a variety of cell fate decisions during development and differentiation of metazoan organisms. On the other hand, dysregulation of Notch1 signaling is associated with many different types of cancer as well as tumor angiogenesis, making Notch1 a potential therapeutic target.

Principal findings

Here we report the in vitro activities of inhibitory Notch1 monoclonal antibodies derived from cell-based and solid-phase screening of a phage display library. Two classes of antibodies were found, one directed against the EGF-repeat region that encompasses the ligand-binding domain (LBD), and the second directed against the activation switch of the receptor, the Notch negative regulatory region (NRR). The antibodies are sel

SUBMITTER: Aste-Amezaga M 

PROVIDER: S-EPMC2817004 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature