Enhancing the longevity of microparticle-based glucose sensors towards 1 month continuous operation.

ABSTRACT: Luminescent microspheres encapsulating glucose oxidase have recently been reported as potential implantable sensors, but the operational lifetime of these systems has been limited by enzyme degradation. We report here that the longevity of these enzymatic microparticle-based sensors has been extended by the coimmobilization of glucose oxidase (GOx) and catalase (CAT) into the sensor matrix. A mathematical model was used to compare the response and longevity of the sensors with and without catalase. To experimentally test the longevity, sensors were continuously operated under normoglycemic dermal substrate concentrations and physiological conditions (5.5 mM glucose and 140 microM O(2), 37 degrees C and pH 7.4). The sensors incorporating CAT were experimentally shown to be approximately 5 t

SUBMITTER: Singh S 

PROVIDER: S-EPMC2818267 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature