UV-induced association of the CSB remodeling protein with chromatin requires ATP-dependent relief of N-terminal autorepression.

ABSTRACT: The ATP-dependent chromatin remodeler CSB is essential for transcription-coupled DNA repair, and mutations in CSB lead to Cockayne syndrome. Here, we examined the recruitment of CSB to chromatin after ultraviolet (UV) irradiation and uncovered a regulatory mechanism that ensures the specific association of this remodeler with chromatin. We demonstrate that ATP hydrolysis by CSB is essential for stable CSB-chromatin association after UV irradiation and that defects in this association underlie some forms of Cockayne syndrome. We also show that the N-terminal region of CSB negatively regulates chromatin association during normal cell growth. Of interest, in the absence of the negative regulatory region, ATP hydrolysis becomes dispensable for chromatin association, indicating that CSB uses en

SUBMITTER: Lake RJ 

PROVIDER: S-EPMC2818792 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature