Growth factor regulation of corneal keratocyte differentiation and migration in compressed collagen matrices.

ABSTRACT:

Purpose

To evaluate a novel 3D culture model of the corneal stroma and apply it to investigate how key wound-healing growth factors regulate the mechanics of corneal keratocyte migration.

Methods

Rabbit corneal keratocytes were seeded within collagen matrices that were compacted using external compression. Six-millimeter-diameter buttons were then incubated in media supplemented with 10% FBS, TGFbeta1, TGFbeta2, platelet-derived growth factor (PDGF), or no growth factor (control). After 1, 3, or 7 days, matrices were labeled with phalloidin and a nucleic acid dye, and were imaged using laser confocal microscopy. To study cell migration, buttons were nested within acellular uncompressed outer collagen matrices before growth factor stimulation.

Results

Corneal keratocyt

SUBMITTER: Kim A 

PROVIDER: S-EPMC2819331 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature