Lsh mediated RNA polymerase II stalling at HoxC6 and HoxC8 involves DNA methylation.

ABSTRACT: DNA cytosine methylation is an important epigenetic mechanism that is involved in transcriptional silencing of developmental genes. Several molecular pathways have been described that interfere with Pol II initiation, but at individual genes the molecular mechanism of repression remains uncertain. Here, we study the molecular mechanism of transcriptional regulation at Hox genes in dependence of the epigenetic regulator Lsh that controls CpG methylation at selected Hox genes. Wild type cells show a nucleosomal deprived region around the transcriptional start site at methylated Hox genes and mediate gene silencing via Pol II stalling. Hypomethylation in Lsh-/- cells is associated with efficient transcriptional elongation and splicing, in part mediated by the chromodomain protein Chd1. Dynami

SUBMITTER: Tao Y 

PROVIDER: S-EPMC2820093 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature