Quantitative analysis of the immune response upon Salmonella typhimurium infection along the porcine intestinal gut.

ABSTRACT: Salmonella enterica serovar Typhimurium causes enteric disease and compromises food safety. In pigs, the molecular response of the intestine to S. typhimurium has been traditionally characterized by in vitro models that do not reflect the actual immunological competence of the intestinal mucosa. In this work, we performed an oral S. typhimurium infection study to obtain insight into the in vitro response in three different sections (jejunum, ileum and colon) of the porcine intestine. For this, samples from one-month-old infected piglets were collected during a time course comprising 1, 2, and 6 days post inoculation to evaluate the intestinal response by quantifying the mRNA expression of gene coding for 28 innate immune system molecules using quantitative real-time PCR assays. In addition

SUBMITTER: Collado-Romero M 

PROVIDER: S-EPMC2820228 | biostudies-literature | 2010 Mar-Apr

REPOSITORIES: biostudies-literature