Establishing new sites of polarization by microtubules.

ABSTRACT:

Background

Microtubules (MTs) participate in the spatial regulation of actin-based processes such as cytokinesis and cell polarization. The fission yeast Schizosaccharomyces pombe is a rod-shaped cell that exhibits polarized cell growth at cell tips. MT plus ends contact and shrink from the cell tips and contribute to polarity regulation.

Results

Here, we investigate the effects of changing cell shape on MTs and cell-polarization machinery. We physically bend fission yeast cells by forcing them into microfabricated femtoliter chambers. In these bent cells, MTs maintain a straight axis and contact and shrink from cortical sites at the sides of cells. At these ectopic sites, polarity factors such as bud6p, for3p (formin), and cdc42p are recruited and assemble actin cables in a

SUBMITTER: Minc N 

PROVIDER: S-EPMC2820583 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature