Downregulation of a chitin deacetylase-like protein in response to baculovirus infection and its application for improving baculovirus infectivity.

ABSTRACT: Several expressed sequence tags (ESTs) with homology to chitin deacetylase-like protein (CDA) were selected from a group of Helicoverpa armigera genes whose expression changed after infection with H. armigera single nucleopolyhedrovirus (HearNPV). Some of these ESTs coded for a midgut protein containing a chitin deacetylase domain (CDAD). The expressed protein, HaCDA5a, did not show chitin deacetylase activity, but it showed a strong affinity for binding to chitin. Sequence analysis showed the lack of any chitin binding domain, described for all currently known peritrophic membrane (PM) proteins. HaCDA5a has previously been detected in the H. armigera PM. Such localization, together with its downregulation after pathogen infection, led us to hypothesize that this protein might be responsib

SUBMITTER: Jakubowska AK 

PROVIDER: S-EPMC2820900 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature