The latent origin of replication of Epstein-Barr virus directs viral genomes to active regions of the nucleus.

ABSTRACT: The Epstein-Barr virus efficiently infects human B cells. The EBV genome is maintained extrachromosomally and replicates synchronously with the host's chromosomes. The latent origin of replication (oriP) guarantees plasmid stability by mediating two basic functions: replication and segregation of the viral genome. While the segregation process of EBV genomes is well understood, little is known about its chromatin association and nuclear distribution during interphase. Here, we analyzed the nuclear localization of EBV genomes and the role of functional oriP domains FR and DS for basic functions such as the transformation of primary cells, their role in targeting EBV genomes to distinct nuclear regions, and their association with epigenetic domains. Fluorescence in situ hybridization visuali

SUBMITTER: Deutsch MJ 

PROVIDER: S-EPMC2820910 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature