Compartmentalization and clonal amplification of HIV-1 variants in the cerebrospinal fluid during primary infection.

ABSTRACT: Human immunodeficiency virus type 1 (HIV-1)-associated dementia (HAD) is a severe neurological disease that affects a subset of HIV-1-infected individuals. Increased compartmentalization has been reported between blood and cerebrospinal fluid (CSF) HIV-1 populations in subjects with HAD, but it is still not known when compartmentalization arises during the course of infection. To assess HIV-1 genetic compartmentalization early during infection, we compared HIV-1 populations in the peripheral blood and CSF in 11 primary infection subjects, with analysis of longitudinal samples over the first 18 months for a subset of subjects. We used heteroduplex tracking assays targeting the variable regions of env and single-genome amplification and sequence analysis of the full-length env gene to identi

SUBMITTER: Schnell G 

PROVIDER: S-EPMC2820937 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature