Optimization of RNA extraction for PCR quantification of aromatic compound degradation genes.

ABSTRACT: Seven different bacterial strains and primer sets and a mixed community were used to evaluate the use of reverse transcriptase quantitative PCR (Q-PCR) and Q-PCR of oxygenase genes to assess various approaches for monitoring the bioremediation of polluted sites. Differences in maximum activity were seen when different RNA extraction kits were compared.

SUBMITTER: Kong W 

PROVIDER: S-EPMC2820950 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature