A primary xenograft model of small-cell lung cancer reveals irreversible changes in gene expression imposed by culture in vitro.

ABSTRACT: Traditional approaches to the preclinical investigation of cancer therapies rely on the use of established cell lines maintained in serum-based growth media. This is particularly true of small-cell lung cancer (SCLC), where surgically resected tissue is rarely available. Recent attention has focused on the need for better models that preserve the integrity of cancer stem cell populations, as well as three-dimensional tumor-stromal interactions. Here we describe a primary xenograft model of SCLC in which endobronchial tumor specimens obtained from chemo-naive patients are serially propagated in vivo in immunodeficient mice. In parallel, cell lines grown in conventional tissue culture conditions were derived from each xenograft line, passaged for 6 months, and then reimplanted to generate se

SUBMITTER: Daniel VC 

PROVIDER: S-EPMC2821899 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature