Comparative metagenomic analysis of plasmid encoded functions in the human gut microbiome.

ABSTRACT:

Background

Little is known regarding the pool of mobile genetic elements associated with the human gut microbiome. In this study we employed the culture independent TRACA system to isolate novel plasmids from the human gut microbiota, and a comparative metagenomic analysis to investigate the distribution and relative abundance of functions encoded by these plasmids in the human gut microbiome.

Results

Novel plasmids were acquired from the human gut microbiome, and homologous nucleotide sequences with high identity (>90%) to two plasmids (pTRACA10 and pTRACA22) were identified in the multiple human gut microbiomes analysed here. However, no homologous nucleotide sequences to these plasmids were identified in the murine gut or environmental metagenomes. Functions encoded by th

SUBMITTER: Jones BV 

PROVIDER: S-EPMC2822762 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature