Characterization of differential properties of rabbit tendon stem cells and tenocytes.

ABSTRACT:

Background

Tendons are traditionally thought to consist of tenocytes only, the resident cells of tendons; however, a recent study has demonstrated that human and mouse tendons also contain stem cells, referred to as tendon stem/progenitor cells (TSCs). However, the differential properties of TSCs and tenocytes remain largely undefined. This study aims to characterize the properties of these tendon cells derived from rabbits.

Methods

TSCs and tenocytes were isolated from patellar and Achilles tendons of rabbits. The differentiation potential and cell marker expression of the two types of cells were examined using histochemical, immunohistochemical, and qRT-PCR analysis as well as in vivo implantation. In addition, morphology, colony formation, and proliferation of TSCs and te

SUBMITTER: Zhang J 

PROVIDER: S-EPMC2822826 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature