Neuropilin-1 antagonism in human carcinoma cells inhibits migration and enhances chemosensitivity.

ABSTRACT:

Background

Neuropilin-1 (NRP1) is a non-tyrosine kinase receptor for vascular endothelial growth factor (VEGF) recently implicated in tumour functions.

Methods

In this study we used a specific antagonist of VEGF binding to the NRP1 b1 domain, EG3287, to investigate the functional roles of NRP1 in human carcinoma cell lines, non-small-cell lung A549, kidney ACHN, and prostate DU145 cells expressing NRP1, and the underlying mechanisms involved.

Results

EG3287 potently displaced the specific binding of VEGF to NRP1 in carcinoma cell lines and significantly inhibited the migration of A549 and ACHN cells. Neuropilin-1 downregulation by siRNA also decreased cell migration. EG3287 reduced the adhesion of A549 and ACHN cells to extracellular matrix (ECM), and enhanced the ant

SUBMITTER: Jia H 

PROVIDER: S-EPMC2822953 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature