YabA of Bacillus subtilis controls DnaA-mediated replication initiation but not the transcriptional response to replication stress.

ABSTRACT: yabA encodes a negative regulator of replication initiation in Bacillus subtilis and homologues are found in many other gram-positive species. YabA interacts with the beta-processivity clamp (DnaN) of DNA polymerase and with the replication initiator and transcription factor DnaA. Because of these interactions, YabA has been proposed to modulate the activity of DnaA. We investigated the role of YabA in regulating replication initiation and the activity of DnaA as a transcription factor. We found that YabA function is mainly limited to replication initiation at oriC. Loss of YabA did not significantly alter expression of genes controlled by DnaA during exponential growth or after replication stress, indicating that YabA is not required for modulating DnaA transcriptional activity. We also f

SUBMITTER: Goranov AI 

PROVIDER: S-EPMC2823125 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature