Ontology highlight
ABSTRACT:
SUBMITTER: Vellaichamy A
PROVIDER: S-EPMC2823583 | biostudies-literature | 2010 Feb
REPOSITORIES: biostudies-literature
Analytical chemistry 20100201 4
Despite the availability of ultra-high-resolution mass spectrometers, methods for separation and detection of intact proteins for proteome-scale analyses are still in a developmental phase. Here we report robust protocols for online LC-MS to drive high-throughput top-down proteomics in a fashion similar to that of bottom-up proteomics. Comparative work on protein standards showed that a polymeric stationary phase led to superior sensitivity over a silica-based medium in reversed-phase nanocapill ...[more]