Microcrystallography using single-bounce monocapillary optics.

ABSTRACT: X-ray microbeams have become increasingly valuable in protein crystallography. A number of synchrotron beamlines worldwide have adapted to handling smaller and more challenging samples by providing a combination of high-precision sample-positioning hardware, special visible-light optics for sample visualization, and small-diameter X-ray beams with low background scatter. Most commonly, X-ray microbeams with diameters ranging from 50 microm to 1 microm are produced by Kirkpatrick and Baez mirrors in combination with defining apertures and scatter guards. A simple alternative based on single-bounce glass monocapillary X-ray optics is presented. The basic capillary design considerations are discussed and a practical and robust implementation that capitalizes on existing beamline hardware is p

SUBMITTER: Gillilan RE 

PROVIDER: S-EPMC2823584 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature