Normal colon epithelium: a dataset for the analysis of gene expression and alternative splicing events in colon disease.

ABSTRACT:

Background

Studies using microarray analysis of colorectal cancer have been generally beleaguered by the lack of a normal cell population of the same lineage as the tumor cell. One of the main objectives of this study was to generate a reference gene expression data set for normal colonic epithelium which can be used in comparisons with diseased tissues, as well as to provide a dataset that could be used as a baseline for studies in alternative splicing.

Results

We present a dependable expression reference data set for non-neoplastic colonic epithelial cells. An enriched population of fresh colon epithelial cells were obtained from non-neoplastic, colectomy specimens and analyzed using Affymetrix GeneChip EXON 1.0 ST arrays. For demonstration purposes, we have compared the d

SUBMITTER: Mojica W 

PROVIDER: S-EPMC2823691 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature