Well-defined block copolymers for gene delivery to dendritic cells: probing the effect of polycation chain-length.

ABSTRACT: The development of safe and efficient polymer carriers for DNA vaccine delivery requires mechanistic understanding of structure-function relationship of the polymer carriers and their interaction with antigen-presenting cells. Here we have synthesized a series of diblock copolymers with well-defined chain-length using atom transfer radical polymerization and characterized the influence of polycation chain-length on the physico-chemical properties of the polymer/DNA complexes as well as the interaction with dendritic cells. The copolymers consist of a hydrophilic poly(ethylene glycol) block and a cationic poly(aminoethyl methacrylate) (PAEM) block. The average degree of polymerization (DP) of the PAEM block was varied among 19, 39, and 75, with nearly uniform distribution. With increasing P

SUBMITTER: Tang R 

PROVIDER: S-EPMC2823989 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature