Marked deposition of eosinophil-derived neurotoxin in adult patients with eosinophilic esophagitis.

ABSTRACT:

Objectives

Eosinophilic esophagitis (EoE) is characterized by infiltration of eosinophils into esophageal epithelium. Blood levels of an eosinophil granule protein, eosinophil-derived neurotoxin (EDN), have been proposed as a biomarker for EoE. However, information regarding localization of EDN in the diseased tissues has not been available. The goal of this study was to evaluate the magnitude and distribution of EDN deposition in tissue specimens from the esophagus of EoE patients.

Methods

We studied specimens from 10 adult EoE patients and eight histologically normal controls (three under age 17). Sections from mid-esophageal biopsy specimens were stained for EDN by immunofluorescence, using a polyclonal rabbit antibody to EDN. Cellular staining (i.e., infiltration of inta

SUBMITTER: Kephart GM 

PROVIDER: S-EPMC2824254 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature