Microfluidic devices integrating microcavity surface-plasmon-resonance sensors: glucose oxidase binding-activity detection.

ABSTRACT: We have developed miniature (approximately 1 microm diameter) microcavity surface-plasmon-resonance sensors (MSPRS), integrated them with microfluidics, and tested their sensitivity to refractive-index changes. We tested their biosensing capability by distinguishing the interaction of glucose oxidase (M(r) 160 kDa) with its natural substrate (beta-D-glucose, M(r) 180 Da) from its interactions with nonspecific substrates (L-glucose, D-mannose, and 2-deoxy-D-glucose). We ran the identical protocol we had used with the MSPRS on a Biacore 3000 instrument using their bare gold chip. Only the MSPRS was able to detect beta-D-glucose binding to glucose oxidase. Each MSPRS can detect the binding to its surface of fewer than 35,000 glucose oxidase molecules (representing 9.6 fg or 60 zmol of protein

SUBMITTER: Amarie D 

PROVIDER: S-EPMC2824604 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature