Caspase 8 and maspin are downregulated in breast cancer cells due to CpG site promoter methylation.

ABSTRACT:

Background

Epigenetic changes associated with promoter DNA methylation results in silencing of several tumor suppressor genes that lead to increased risk for tumor formation and for progression of the cancer.

Methods

Methylation specific PCR (MSP) and bisulfite sequencing were used for determination of proapoptotic gene Caspase 8 (CASP8) and the tumor suppressor gene maspin promoter methylation in four breast cancer and two non-tumorigenic breast cell lines. Involvement of histone H3 methylation in those cell lines were examined by CHIP assay.

Results

The CpG sites in the promoter region of CASP8 and maspin were methylated in all four breast cancer cell lines but not in two non-tumorigenic breast cell lines. Demethylation agent 5-aza-2'-deoxycytidine (5-aza-dc) select

SUBMITTER: Wu Y 

PROVIDER: S-EPMC2824712 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature