The dynamic architecture of the metabolic switch in Streptomyces coelicolor.

ABSTRACT:

Background

During the lifetime of a fermenter culture, the soil bacterium S. coelicolor undergoes a major metabolic switch from exponential growth to antibiotic production. We have studied gene expression patterns during this switch, using a specifically designed Affymetrix genechip and a high-resolution time-series of fermenter-grown samples.

Results

Surprisingly, we find that the metabolic switch actually consists of multiple finely orchestrated switching events. Strongly coherent clusters of genes show drastic changes in gene expression already many hours before the classically defined transition phase where the switch from primary to secondary metabolism was expected. The main switch in gene expression takes only 2 hours, and changes in antibiotic biosynthesis genes are

SUBMITTER: Nieselt K 

PROVIDER: S-EPMC2824715 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature