Laser microdissection of sensory organ precursor cells of Drosophila microchaetes.

ABSTRACT:

Background

In Drosophila, each external sensory organ originates from the division of a unique precursor cell (the sensory organ precursor cell or SOP). Each SOP is specified from a cluster of equivalent cells, called a proneural cluster, all of them competent to become SOP. Although, it is well known how SOP cells are selected from proneural clusters, little is known about the downstream genes that are regulated during SOP fate specification.

Methodology/principal findings

In order to better understand the mechanism involved in the specification of these precursor cells, we combined laser microdissection, toisolate SOP cells, with transcriptome analysis, to study their RNA profile. Using this procedure, we found that genes that exhibit a 2-fold or greater expression in SOPs

SUBMITTER: Buffin E 

PROVIDER: S-EPMC2824816 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature