Prosaposin down-modulation decreases metastatic prostate cancer cell adhesion, migration, and invasion.

ABSTRACT:

Background

Factors responsible for invasive and metastatic progression of prostate cancer (PCa) remain largely unknown. Previously, we reported cloning of prosaposin (PSAP) and its genomic amplification and/or overexpression in several androgen-independent metastatic PCa cell lines and lymph node metastases. PSAP is the lysosomal precursor of saposins, which serve as activators for lysosomal hydrolases involved in the degradation of ceramide (Cer) and other sphingolipids.

Results

Our current data show that, in metastatic PCa cells, stable down-modulation of PSAP by RNA-interference via a lysosomal proteolysis-dependent pathway decreased beta1A-integrin expression, its cell-surface clustering, and adhesion to basement membrane proteins; led to disassembly of focal adhesion co

SUBMITTER: Hu S 

PROVIDER: S-EPMC2825248 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature